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Chromogenic Endotoxin Quantification Kit for Accurate Bacterial Toxin Measurement

Endotoxins, also known as lipopolysaccharides (LPS), are toxic components of the outer membrane of Gram-negative bacteria. Their presence in pharmaceuticals, medical devices, or water systems can pose serious health risks. Accurate detection and quantification of endotoxins are crucial for ensuring safety in various industries. The Chromogenic Endotoxin Quant Kit provides a reliable and sensitive solution for measuring bacterial endotoxins with precision.

Why Choose the Chromogenic Endotoxin Quant Kit?

The Chromogenic Endotoxin Quant Kit utilizes a chromogenic assay based on the Limulus Amebocyte Lysate (LAL) principle. This method offers several advantages:

• High Sensitivity: Detects endotoxin levels as low as 0.005 EU/mL.
• Accuracy: Provides quantitative results with excellent reproducibility.
• Time Efficiency: Delivers results within 30-60 minutes, depending on the protocol.
• Broad Applicability: Suitable for testing pharmaceuticals, biologics, medical devices, and water samples.

How the Kit Works

The assay involves the activation of a pro-clotting enzyme in the presence of endotoxins, leading to the cleavage of a chromogenic substrate. The resulting color change is proportional to the endotoxin concentration and can be measured spectrophotometrically at 405 nm.

The kit includes:

• LAL reagent reconstituted with chromogenic substrate
• Endotoxin standard for calibration
• Control standard endotoxin (CSE)
• Sample dilution buffer
• Detailed protocol for easy implementation

Applications of Endotoxin Testing

The Chromogenic Endotoxin Quant Kit is widely used in:

• Pharmaceutical Industry: Ensuring the safety of injectable drugs and vaccines.
• Medical Device Manufacturing: Validating the sterility of implants and surgical tools.
• Water Quality Control: Monitoring endotoxin levels in dialysis water and other critical systems.
• Research Laboratories: Studying bacterial pathogenesis and immune responses.

Conclusion

Accurate endotoxin quantification is essential for compliance with regulatory standards such as USP and EP 2.6.14. The Chromogenic Endotoxin Quant Kit offers a robust, user-friendly, and highly sensitive method for detecting bacterial toxins, making it an indispensable tool for quality control and research applications.

For more information on this kit or to request a quote, please contact our technical support team.